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πŸ”₯+ methods developing food products instructional pdf ac30ffb11 30 May 2020 There are several types of diabetes; I'm going to discuss the two main types: type 1 and type 2. Type 1 formerly called juvenile onset diabetes ...

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Clin Hemorheol Microcirc. 2016;64(4):655-662. doi: 10.3233/CH-168021.

Author information

1
Department of Anesthesia, Pain Management and Perioperative Medicine, Dalhousie University, Halifax, NS, Canada.
2
Department of Pharmacology, Dalhousie University, Halifax, NS, Canada.
3
Department of Microbiology and Immunology, Dalhousie University, Halifax, NS, Canada.
4
Department of Physiology and Biophysics, Dalhousie University, Halifax, NS, Canada.
5
Department of Biology, Dalhousie University, Halifax, NS, Canada.
6
Department of Medicine, Dalhousie University, Halifax, NS, Canada.

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BACKGROUND:

Destruction of the insulin-producing beta cells in type 1 diabetes (T1D) is induced by invasion of immune cells causing pancreatic inflammation. Cannabidiol (CBD), a phytocannabinoid, derived from the plant, Cannabis sativa, was shown to lower the incidence of diabetes in non-obese diabetic (NOD) mice, an animal model of spontaneous T1D development.

OBJECTIVE:

The goal of this study was to investigate the impact of experimental CBD the 1 last update 30 May 2020 treatment on early pancreatic inflammation in T1D by intravital microscopy (IVM) in NOD mice.The goal of this study was to investigate the impact of experimental CBD treatment on early pancreatic inflammation in T1D by intravital microscopy (IVM) in NOD mice.

METHODS:

Seven-week-old female NOD mice were prophylactically administered daily 5β€Šmg/kg CBD or control vehicle i.p. five times weekly for ten weeks. Animals underwent IVM following confirmation of T1D diagnosis by blood glucose testing. Leukocyte activation and functional capillary density (FCD) were quantified via IVM.

RESULTS:

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CONCLUSIONS:

Experimental CBD treatment reduced markers of inflammation in the microcirculation of the pancreas studied by intravital microscopy.

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Type 1 diabetes; adhesion molecules; cytokines; functional capillary density; inflammation; intravital microscopy; leukocyte adherence

PMID:
27767974
DOI:
10.3233/CH-168021
[Indexed for MEDLINE]

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